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TFF help for a genetics project (Lake Fork)
#8758658
03/27/13 06:58 PM
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Joined: Mar 2008
Posts: 634
whitneylakerat
OP
Pro Angler
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OP
Pro Angler
Joined: Mar 2008
Posts: 634 |
I am a graduate student at TCU working on a genetics project concerning white and black crappie, the genetic diversity in their populations, and the amount of hybridization that occurs between the two. The good people at bigcrappie.com helped me with tissue samples from Cedar Creek, and now I would like to compare the population structures between the two lakes.
I need a small portion of the tail fin from about fifty different fish and ideally an even mix of black and white crappie. If you are going to be fishing over the next couple of days and could save the tail fins, or a portion of them I could come collect them Friday or Saturday afternoon, or meet you when you are cleaning them.
You can contact me at james.d.hill@tcu.edu or PM me on here to set something up.
Thanks!
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Re: TFF help for a genetics project (Lake Fork)
[Re: whitneylakerat]
#8758794
03/27/13 07:32 PM
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Joined: Feb 2006
Posts: 1,221
CRAPPIEJIGN
Extreme Angler
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Extreme Angler
Joined: Feb 2006
Posts: 1,221 |
What is the best way to save, preserve and maintain the integrity of the tissue for your sampling program?
Inquiring minds want to know.
Jeff
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Re: TFF help for a genetics project (Lake Fork)
[Re: whitneylakerat]
#8758876
03/27/13 07:51 PM
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Joined: Apr 2011
Posts: 2,925
GoldSpoonr
Extreme Angler
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Extreme Angler
Joined: Apr 2011
Posts: 2,925 |
If you feel like a riot Then dont you deny it Put your good foot forward
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Re: TFF help for a genetics project (Lake Fork)
[Re: whitneylakerat]
#8758897
03/27/13 07:56 PM
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Joined: Jun 2012
Posts: 14,308
Ketchn
TFF Guru
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TFF Guru
Joined: Jun 2012
Posts: 14,308 |
hmmmmm freeze em ....maybe ....possibly dry ice ....? i know when they wuz collecting songbirds at our place years ago for sum kinda dna diversity thing .... they had them sum kinda federal permits and .... they packed em in saw dust and dry ice for the long ride back to the university in nevada
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Re: TFF help for a genetics project (Lake Fork)
[Re: whitneylakerat]
#8759006
03/27/13 08:23 PM
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Joined: Feb 2013
Posts: 43
CissyShack
Outdoorsman
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Outdoorsman
Joined: Feb 2013
Posts: 43 |
I happened to be on the dock at bigcrappie when this guy came by. He was clipping the samples from the tails and placing them in a plain letter envelope. I would imagine if you then place them in a freezer then they would maintain the integrity.
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Re: TFF help for a genetics project (Lake Fork)
[Re: whitneylakerat]
#8759060
03/27/13 08:37 PM
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Joined: Mar 2008
Posts: 634
whitneylakerat
OP
Pro Angler
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OP
Pro Angler
Joined: Mar 2008
Posts: 634 |
Great question. CissyShack is correct, what I do is clip them and place them in an envelope to dry.
Sitting on ice would probably be best, but for a day or two any closed container would work. Their fins do not degrade as fast as if I needed a muscle or liver sample. People have done similar studies from scales of preserved specimens in museums that were decades old.
One thing that would be beneficial is to have black and white tail clippings seperate, they could just be put in different plastic sacks
Thanks again
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Re: TFF help for a genetics project (Lake Fork)
[Re: whitneylakerat]
#8761341
03/28/13 12:57 PM
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Joined: Jul 2012
Posts: 3,270
piscatur non solum piscator
TFF Team Angler
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TFF Team Angler
Joined: Jul 2012
Posts: 3,270 |
I know you have a busy schedule but I think you are missing out a bit. Imagine telling folks that you are working and working hard on an important scientific research project. And there you would be, fishing, first on Cedar Creek and then on Fork. It would be a tough job.
Think it over, maybe convince someone to help fund your field research. Hope you get the samples soon!
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Re: TFF help for a genetics project (Lake Fork)
[Re: piscatur non solum piscator]
#8761366
03/28/13 01:03 PM
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Joined: Jan 2013
Posts: 6,950
crapicat
TFF Celebrity
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TFF Celebrity
Joined: Jan 2013
Posts: 6,950 |
I know you have a busy schedule but I think you are missing out a bit. Imagine telling folks that you are working and working hard on an important scientific research project. And there you would be, fishing, first on Cedar Creek and then on Fork. It would be a tough job.
Think it over, maybe convince someone to help fund your field research. Hope you get the samples soon! I'm fairly certain, i could enjoy some of that important scientific field research...hmm, now if I could just remember where to sign up for that research grant money!!!!
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Re: TFF help for a genetics project (Lake Fork)
[Re: whitneylakerat]
#8761426
03/28/13 01:19 PM
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Joined: Apr 2008
Posts: 592
Hickory Nut
Pro Angler
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Pro Angler
Joined: Apr 2008
Posts: 592 |
I will be on Fork Mon thru Thur next week. If there is some grant money to help with gas, food, bait, etc,I will be more than happy to participate:) Seriously, I would be glad to help out, but I see where you are wanting them Fri or Sat. If You can come over later I will be glad to keep some, providing I catch some to keep. Joe,cell 940-631-4241
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